http://www.osumicrobiology.org/homepages/artsimovitch/sequences/pVSXXX.seq

name	description	Ab^R	Seq
pVS4	recreating 312 to attain high induction levels: XhoI to XbaI fragment of  cloned between the same sites of pIA299	Amp	Seq
pVS5	MfeI to ApaI fragment from pIA452 cloned into pIA423	Amp	Seq
pVS6	XbaI-HindIII fragment from pIA446 cloned into LITMUS28	Amp	Seq
pVS7	BglII-XbaI fragment from pVS6 cloned into the same sites of pIA462	Tet	Seq
pVS8	EcoRI^Q111 mutant amplified from pEQ111m with oligos 159 and 160 and cloned between NdeI and SapI sites of pTYB1	Amp	Seq
pVS9	EcoRI^Q111 mutant amplified from pEQ111m with oligos 159 and 160 and cloned between NdeI and XhoI sites of pET33	Kn	Seq
pVS10	E. coli RNAP overexpression vector: all subunits = a,β,β’, and w. His6 tag at the C-terminus of β’	Amp	Seq
pVS11	E.coli dksA in pTYB12, cloned between BsmI and PstI, has an extra A at the N-terminus after cleavage	Amp	Seq
pVS12	ECOLI rfaH in pTYB1, cloned between NdeI and SapI, the C-terminal L is replaced by A, to improve intein cleavage	Amp	Seq
pVS13	VCHOL rfaH in pTYB1, cloned between NdeI and SapI, the C-terminal T is replaced by A, to improve intein cleavage	Amp	Seq
pVS14	β' SI3 deletion in pVS10 - recloned BsmI-HindIII fragment from pIA301	Amp	Seq
pVS15	β ' T790L in pIA299 ; SDM with oligos 327+328	Amp	Seq
pVS16	β ' T790A in pIA299;  SDM with oligos 325+326	Amp	Seq
pVS17	E.coli rpoH cloned between Mva1269I and NotI of pTYB12	Amp	Seq
pVS18	E.coli dksA Asp71Asn,Asp74Asn in pVS11, SDM with oligos 408+409	Amp	Seq
pVS19	E.coli dksA Asp71Ala,Asp74Ala in pVS11, SDM with oligos 410+411	Amp	Seq
pVS20	RfaH domain1/N-term PCR cloned into NdeI-SapI sites of pTYB1 (SapI is gone in the final clone)	Amp	Seq
pVS21	RfaH domain2/C-term PCR cloned into NdeI-SapI sites of pTYB1 (SapI is gone in the final clone)	Amp	Seq
pVS25	T. thermophilus greA: PCR with oligos 513+514 cloned between PstI and BsmI of pTYB12	Amp	Seq
pVS26	T. thermophilus gfhI: PCR with oligos 511+512 cloned between SalI and BsmI of pTYB12	Amp	Seq
pVS28	DbpA in pTYB12	Amp	Seq
pVS29	SrmB in pTYB12	Amp	Seq
pVS35	β ' Y457H: SbfI to BsmI fragment from pIA521 recloned into pVS10	Amp	Seq
pVS36	β ' N458S: SbfI to BsmI fragment from pIA521 recloned into pVS10	Amp	Seq
pVS40	T7A1 - consensus ops -scrambled ops in pIA416	Amp	Seq
pVS46	T7A1 - scrambled ops in pIA416	Amp	Seq
pVS47	YacL in pTYB12: PCR 649-648 cloned between BsmI and SalI sites	Amp	Seq
pVS48	β’ F773V from pIA387 cloned between BsmI and HindIII sites of pVS10; silent SnaBI site	Amp	Seq
pVS49	β’ IS774 (silent EarI site) from pIA388 cloned between BsmI and HindIII sites of pVS10	Amp	Seq
pVS50	β’ 672,673DD  in pVS10	Amp	Seq
pVS51	deletions of the β’ C-terminal CC residues 665-671and 674-680 in pVS10	Amp	Seq
pVS53	T7A1 - consensus ops - consensus ops in pIA416	Amp	Seq
pVS54	T7A1 - scrambled ops - scrambled ops in pIA416	Amp	Seq
pVS55	T7A1  - scrambled ops - consensus ops in pIA416	Amp	Seq
pVS56	RfaH E. coli + HMK (C-term): genomic PCR 58-746 cloned into NdeI and SapI of pTYB1	Amp	Seq
pVS57	α-His6β (D226-350)- β’: moved NcoI-SbfI fragment from pIA319 (rifS) into pIA679	Amp	Seq
pVS58	T. thermophilus NusG cloned between NdeI and NotI sites of pTYB12;genomic PCR with oligos 392+742	Amp	Seq
pVS60	T. thermophilus RpoD in pTYB12 714+715	Amp	Seq
pVS61	RfaH R23A in pIA432; silent XhoI site	Kn	Seq
pVS62	RfaH R73D in pIA432; SacII site disappears (SDM with 930+931)	Kn	Seq
pVS63	MTUBE RbpA in pTYB12: genomic PCR with 894+895 cloned into BsmI and PstI of pTYB12	Amp	Seq
pVS66	RfaH R73A in pIA432; SacII site disappears	Kn	Seq
pVS67	RfaH M140D in pIA432; silent BglII site	Kn	Seq
pVS68	RfaH L143Din pIA432; no sites	Kn	Seq
pVS69	ActX in pTYB12: genomic PCR w/883+884 cloned into BsmI and PstI of pTYB12	Amp	Seq
pVS101	E.coli crl in pTYB12 genomic PCR with 988+989 cloned into Mva1269I and PstI	Amp	Seq
pVS103	RfaH pIA432::H152I (PCR 92+1074)	Kn	Seq
pVS104	RfaH pIA432::A122P (1075+1076)	Kn	Seq
pVS109	GreB T141E in pIA576; SDM with oligos 1135+1136	Kn	Seq
pVS110	GreB S122E in pIA576; SDM with oligos 1133+1134	Kn	Seq
pVS111	GreB M124E in pIA576; SDM with oligos 1131+1132	Kn	Seq
pVS112	GreB I118E in pIA576; SDM with oligos 1137+1138	Kn	Seq