This page is under perpetual construction. Not yet hyperlinked sequences of pIA plasmids can often be downloaded from this address:
http://www.osumicrobiology.org/homepages/artsimovitch/sequences/pIAXXX.seq
where XXX is the plasmid number.
|
name |
Description
|
AbR |
Seq |
|
pIA140
|
PL-nutL-tR’ |
Amp |
|
|
pIA142
|
T7A1-nutR-tR’ |
Amp |
|
|
pIA144
|
PL-nutL-hisT |
Amp |
|
|
pIA146
|
ClaI-BspEI fragment from pRL574 cloned into pRL777:T7A1, A29, no terminator in sight |
Amp |
|
|
pIA148 |
ClaI-BspEI fragment from pRL574 cloned into pRM143: lPR, C135, no terminator in sight
|
Amp |
|
|
pIA150
|
Deletion of aa 938-1040 in E. coli b in pRL702 |
Amp |
|
|
pIA160
|
XhoI site in E. coli b at 998 in pRL702 |
Amp |
|
|
pIA161
|
2H1 fragment (rpoC N-term.) cloned between BamHI and PstI sites of pGAD424 |
Amp |
|
|
pIA162
|
2H2 fragment (rpoC middle) cloned between BamHI and PstI sites of pGAD424 |
Amp |
|
|
pIA163
|
2H3 fragment (rpoC C-term.) cloned between BamHI and PstI sites of pGAD424 |
Amp |
|
|
pIA164
|
2H4 fragment (rpoB N-term.) cloned between BamHI and PstI sites of pGAD424 |
Amp |
|
|
pIA165
|
2H5 fragment (rpoB middle) cloned between BamHI and PstI sites of pGAD424 |
Amp |
|
|
pIA166
|
2H6 fragment (rpoB C-term.) cloned between BamHI and PstI sites of pGAD424 |
Amp |
|
|
pIA167 |
UUUG tetraloop in pCL102b
|
Amp |
|
|
pIA168
|
UUUG tetraloop in pCL102b |
Amp |
|
|
pIA169
|
UGA in place of Cys77 codon of lacZ in pRL602 |
Amp |
|
|
pIA170
|
UGA in place of Cys77 codon of lacZ in pIA12 |
Amp |
|
|
pIA171
|
WT downstream sequence combined with the A29 complex from pCL102b |
Amp |
|
|
pIA172
|
-10, -9, -8 TTT substitution in pIA171 |
Amp |
|
|
pIA173
|
-10, -9, -8 TTT substitution combined with GG insertion in the hairpin in pIA171 |
Amp |
|
|
pIA174
|
deletion of E. coli b aa 900-909 in pSJ103; also contains a Ala875Pro substitution; introduces extra BspEI site |
Amp |
|
|
pIA175
|
G to A substitution at the his pause site in pIA171 |
Amp |
|
|
pIA176
|
E. coli b F906A in pIA178 |
Amp |
|
|
pIA177
|
E. coli b F934A in pIA178 |
Amp |
|
|
pIA178
|
E. coli b rifd18(S531F) + 998 XhoI in pRL702 |
Amp |
|
|
pIA179
|
E. coli b K909D in pIA178 |
Amp |
|
|
pIA180
|
E. coli b K909D, D516V in pIA160 |
Amp |
|
|
pIA181
|
E. coli b D900-909 in pIA178 |
Amp |
|
|
pIA182
|
E. coli b D900-909, D516V in pIA160 |
Amp |
|
|
pIA183
|
E. coli b D516V in pIA160 |
Amp |
|
|
pIA184
|
E. coli b’ 762A, 763A, 764A in pRL663 |
Amp |
|
|
pIA185
|
WT put in pIA171 |
Amp |
|
|
pIA186
|
D put in pIA171 |
Amp |
|
|
pIA187
|
E. coli b’ 762A, 763A, 764A in pRL662 |
Amp |
|
|
pIA188
|
his71B in pIA171 |
Amp |
|
|
pIA189
|
his71C in pIA171 |
Amp |
|
|
pIA190
|
his71C+mutant hairpin in pIA171 |
Amp |
|
|
pIA191
|
l PR in pIA170 |
Amp |
|
|
pIA192
|
E. coli bK909A in pIA183 |
Amp |
|
|
pIA193
|
E. coli bK909S in pIA183 |
Amp |
|
|
pIA194
|
E. coli bK909G in pIA183 |
Amp |
|
|
pIA195
|
E. coli bK909P in pIA183 |
Amp |
|
|
pIA196
|
E. coli bX29Lys6 in pIA183 |
Amp |
|
|
pIA197
|
E. coli bF906A in pIA183 |
Amp |
|
|
pIA198
|
E. coli bF934A in pIA183 |
Amp |
|
|
pIA199
|
B. subtilis P RNA in pCL185 – makes G16 |
Amp |
|
|
pIA200
|
l N cloned between NdeI and XhoI sites of pET21b - his6 tag at the 3' end. |
Amp |
|
|
pIA201
|
l N cloned between NdeI and XhoI sites of pET21b |
Amp |
|
|
pIA202
|
-13 through -9 AATTT substitution in pIA171 |
Amp |
|
|
pIA203
|
mutation in pIA204 - D of C between HphI and SpeI sites |
Amp |
|
|
pIA204
|
his perfect hairpin in pIA171 |
Amp |
|
|
pIA205
|
E. coli bF934A, S531F in pRW408 |
Amp |
|
|
pIA206
|
E. coli bK909D, S531F in pRW408 |
Amp |
|
|
pIA207
|
E. coli bD 900-909, S531F in pRW408 |
Amp |
|
|
pIA208
|
E. coli bK909A, S531F in pRW408 |
Amp |
|
|
pIA209
|
E. coli bK909S, S531F in pRW408 |
Amp |
|
|
pIA210
|
E. coli bF906A, S531F in pRW408 |
Amp |
|
|
pIA211
|
l to cloned between BglII and BamHI sites of pIA214 |
Amp |
|
|
pIA212
|
trpP constitutive promoter cloned between EcoRI and BglII of pIA175 |
Amp |
|
|
pIA213
|
promoter-less CAT gene cloned into EcoRV site of pET28a |
Amp |
|
|
pIA214
|
promoter-less CAT gene + US cloned between XbaI and HindIII sites of pIA212 |
Amp |
|
|
pIA215
|
l to cloned between BglII and BamHI sites of pIA214 |
Amp |
|
|
pIA216
|
tR' cloned between BglII and XbaI sites of pIA214 |
Amp |
|
|
pIA217
|
T7Te cloned between BglII and XbaI sites of pIA214 |
Amp |
|
|
pIA218
|
perfect hairpin that extends to the -10 position from the 3’ end in pIA171 |
Amp |
|
|
pIA219
|
perfect hairpin that extends to the -9 position from the 3’ end in pIA171 |
Amp |
|
|
pIA220
|
perfect hairpin that extends to the -8 position from the 3’ end in pIA171 |
Amp |
|
|
pIA221
|
perfect hairpin that extends to the -7 position from the 3’ end in pIA171 |
Amp |
|
|
pIA222
|
E. coli bD516V with silent BsgI site - original isolate from Quick Change |
Amp |
|
|
pIA223
|
E. coli bD516V with BsgI site - ClaI to BspEI fragment of pIA222 cloned into pIA160 |
Amp |
|
|
pIA224
|
Mutant 3' proximal region (UUU->ccc) in T7 terminator in pCM301 |
Amp |
|
|
pIA225
|
Mutant 3' proximal region (UCU->cuc) in T7 terminator in pCM301 |
Amp |
|
|
pIA226
|
hisP signal cloned under control of l Pr between BbsI and SphI sites of pCM301 |
Amp |
|
|
pIA227
|
D of E. coli b’ in pRM339 |
Amp |
|
|
pIA228
|
his pause signal cloned under control of l Pr promoter between BbsI and SphI of pCM301 |
Amp |
|
|
pIA229
|
his perfect hairpin cloned under control of l Pr promoter between BbsI and SphI of pCM301 |
Amp |
|
|
pIA230
|
D of NsiI-MfeI fragment (= cI repressor) in pIA229 |
Amp |
|
|
pIA231
|
NsiI-HindIII fragment from pIA227 into NsiI-HindIII of pBAD33. |
Cm |
|
|
pIA232
|
PCR 3430+3431 from pRL719 cloned between PstI &EcoRI of pBR322 - new StuI site & bla is deleted |
Tet only |
|
|
pIA233
|
killed XhoI site at 10320 in pRL719 |
Amp |
|
|
pIA234
|
SgfI(T4) fragment of pRL719 cloned between SmaI and HincII sites of pBSIIKS-: reverse |
Amp |
|
|
pIA235
|
SgfI(T4) fragment of pRL719 cloned between SmaI and HincII sites of pBSIIKS- |
Amp |
|
|
pIA236
|
NsiI to StuI fragment (9806 bp) of pRL719 cloned into NsiI & StuI sites of pIA232 |
Tet |
|
|
pIA237
|
ops site from rfaS cloned between SpeI and BglII sites of pIA171 |
Amp |
|
|
pIA238
|
E. coli rfaH cloned between NdeI and HindIII sites of pET28a |
Kn |
|
|
pIA239
|
S.cerevisiae rpb2 Zn-finger region cloned between Sse87I and HindIII sites of pIA178 |
Amp |
|
|
pIA240
|
S.cerevisiae rpb2 Zn-finger region cloned between Sse87I and HindIII sites of pIA233 |
Amp |
|
|
pIA241
|
Derivative of pCM301: change in the G26 to remove tandem Us + NheI site |
Amp |
|
|
pIA242
|
XbaI site at E. coli b' aa103 in pIA235 - recloned SseI to MluI fragment from pIA243 into pIA235 |
Amp |
|
|
pIA243
|
XbaI site at E. coli b' aa103 in pIA235 - original isolate from Quick Change mutagenesis |
Amp |
|
|
pIA244
|
E. coli NusG in pET33 between NdeI and HindIII; His6 tag + HMK site at the N-terminus |
Kn |
|
|
pIA245
|
E. coli NusG in pET21b between NdeI and HindIII - has a frameshift at the 3’ end + His6 tag |
Amp |
|
|
pIA246
|
E. coli NusG in pET21b between NdeI and HindIII |
Amp |
|
|
pIA247
|
E. coli NusG in pET28a between NdeI and HindIII: His6 tag at the N-terminus |
Kn |
|
|
pIA248
|
His6-NusG in the Ptrc plasmid - NcoI-HindIII fragment of pIA247 cloned into pRW308 |
Amp |
|
|
pIA249
|
His6-RfaH in the Ptrc plasmid - NcoI-HindIII fragment of pIA238 cloned into pRW308 |
Amp |
|
|
pIA250
|
137AT in B. subtilis P RNA – made from pIA199 by Quick change mutagenesis |
Amp |
|
|
pIA251
|
ops site from pheP cloned between SpeI and BglII sites of pIA171 |
Amp |
|
|
pIA252
|
137AT in B. subtilis P RNA – made from pCP240 by Quick change mutagenesis |
Amp |
|
|
pIA253
|
T8 in pCM301 between BbsI and BamHI |
Amp |
|
|
pIA254
|
T9 in pCM301 between BbsI and BamHI |
Amp |
|
|
pIA255
|
his terminator in pIA241. PCR 3495-3071 cloned between NheI and BamHI sites of pIA241 |
Amp |
|
|
pIA256
|
E.coli topA gene fragment under T7A1+A29 - random isolate from cloning PCR fragment 3483-3484 from genomic DNA into SpeI and BglII sites of pIA171 |