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Department of Microbiology

 
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The Ohio State University
Facilities and Resources

The OSU Fermentation Facility

Welcome to the OSU Fermentation Facility Home Page. We are a BSL2 service laboratory, supported by the Microbiology Department, located on the main campus of The Ohio State University. This facility provides fermentation services for the College of Biological250 liter Ferm Sciences, the Ohio State campus, and limited off-campus support.

Services provided by Fermentation Lab

  • Batch fermentation for the production of microbial biomass or enzymes.

    -Batch fermentations ranging from 1 to 200 liters in size.

  • Anaerobic and thermophilic fermentations.

    -Batch fermentation and harvesting of thermophilic anaerobic  organisms up to 20 liters.

  • Chemostat and continuous fermentations (with developed protocol)

    -Chemostat type fermentations up to 5 liters.

  • Limited consultation services.

    -Fermentation lab personnel can offer assistance in the following areas:

    a) Seed culture development.
    b) Media optimization.
    c) Chemical antifoam usage and selection.
    d) Cell harvesting and product recovery.
    e) Recommendation of fermentation and cell harvesting equipment.

 

Fees and charges for use of the facility by OSU labs.  These fees reflect a discount to assist OSU faculty in the advancement of their research objectives. Off campus users may inquire calling (614) 292-3277.

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Fees and Charges

Description
Amt /Basis
10 LITER FERMENTOR
FIRST DAY FEE
ADDITIONAL DAY
22.00
5.00 / DAY
200 LITER FERMENTOR
FIRST DAY FEE
ADDITIONAL DAY
300.00
100.00 / DAY
20 LITER FERMENTOR
FIRST DAY FEE
ADDITIONAL DAY

100.00
50.00 / DAY
PELLICON
HARVEST 10 LITERS
HARVEST 20 LITERS
HARVEST 30 LITERS
HARVEST 40 LITERS
HARVEST 50 LITERS
30.00 / FERMENTATION
40.00 / FERMENTATION
50.00 / FERMENTATION
60.00 / FERMENTATION
70.00 / FERMENTATION
HERAEUS
HARVEST 10 LITERS
HARVEST 20 LITERS
HARVEST 30 LITERS
30.00 / FERMENTATION
40.00 / FERMENTATION
50.00 / FERMENTATION
AMICON
HARVEST 190 LITERS
150.00 / FERMENTATION
LABOR 20.00 / HR

Labor is included in the above basic fermentations fees. This fee will be added only when there are additional requests such as manual sampling, monitoring, and sample treatment.

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Schedule a Fermentation

Call (614) 292-3277 and let the manager know the following:

  • If this is your first contact with the fermentation lab.  If not, give the time you last had a fermentation run.
  • Desired volume of the fermentation.
    Specific organism you want to grow and the recommended protocol (if there is one).
  • Total time (estimation of course) of the fermentation.  If you're not sure we can go over the protocol together and schedule it accordingly.  This is mainly for organisms which are new to the lab.
  • If you want the organism to express a protein (recombinant or otherwise) let me know if there are any specific aspects of the fermentation which may be important.
  • The purpose of the fermentation.(optional)

 

Keep in mind that all fermentations must be approved by the manager before any work begins.  Also, we will need all standard media components before noon on the day before the fermentation is scheduled to start.

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Department of Microbiology; The Ohio State University; 376 Bioscience Building; 484 West 12th Ave.; Columbus, Ohio USA; 43210-1292; Phone: 614-292-2301; Fax: 614-292-8120
Riffe Research Center