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Hua Wang

wang.707@osu.edu

 

B.S., Microbiology, Fudan University
Ph.D., Food Science and Nutrition, University of Minnesota
Postdoc, Molecular Biology, NIH/NICHD

 

Associate Professor of Microbiology; Food Science and Technology; Interdisciplinary Ph.D. Program in Human Nutrition
Member, Center for Microbial Interface Biology

Microbial biofilms involving foodborne microorganisms; antibiotic resistance in food and host microbial ecosystem

Dr. Wang's main research interests are microbial biofilms involving foodborne microorganisms, and antibiotic resistance in food and host microbial ecosystems. Her group defined the first "honeycomb" biofilm structure in Listeria monocytogenes (FEMS Microbiol Lett. 2003, 228:203-210), and demonstrated the role of conjugation in microbial biofilm ecosystem development in Gram-positive bacteria using the Lactococcus lactis model system (Appl. Environ. Microbiol. 2005, 71:2970-2978). Her research group also revealed the magnitude of antibiotic resistant commensal (including non-pathogenic and beneficial) bacteria with mobile resistance genes transmissible to human residential bacteria and pathogens in commonly consumed food items, and illustrated the potential role of commensal bacteria as facilitators in horizontal gene transmission. These studies, along with data from other laboratories, suggested that the food chain might have played an important role in the dissemination of antibiotic resistance to humans (Appl. Environ. Microbiol. 2005, 71:2970-2978; FEMS Microbiol Lett. 2006, 254:226-231; Appl. Environ. Microbiol. 2007, 73:1676-1677; http://www.eurekalert.org/pub_releases/2007-05/asfm-rgi051707.php). Her group is currently investigating the contributions of commensal bacteria in the facilitated dissemination, persistence and enrichment of antibiotic resistance in the natural environment, food chain and the hosts.

Recent Publications

Jaykus L, Wang HH, Schlesinger L. (eds). 2009. Foodborne Microbes: Shaping the Host Ecosystems. ASM Press.

Wang HH. 2009. Commensal bacteria, microbial ecosystems and horizontal gene transmission: adjusting our focus for strategic breakthroughs against antibiotic resistance. In Foodborne Microbes: Shaping the Host Ecosystems (Jaykus L, Wang HH, Schlesinger L., eds). ASM Press.

Manuzon MY, Hanna SE, Luo H, Harper WJ, Wang HH. 2007. Development of a real-time PCR method to quantify antibiotic resistance gene pool in retail dairy products. Appl Environ Microbiol. Jan 5; [Epub ahead of print]

Wan K, Yousef AE, Schwartz SJ, Wang HH. 2006. Rapid, specific and sensitive detection of spoilage molds in orange juice using a real-time Taqman ® PCR assay. J Food Protect. 69:385-390.

Wang HH, Manuzon MY, Lehman M, Wan K, Luo H, Wittum TE, Yousef AE, Bakaletz LO. 2006. Food commensal microbes as a potentially important avenue in transmitting antibiotic resistance genes. FEMS Micrbiol Lett. 254: 226-231. Erratum in: FEMS Microbiol Lett. 255:328.

Lu L, Tomita A, Wang H, Buchholz DR, Shi YB. 2006. Transcriptional regulation of the Xenopus laevis stromelysin-3 gene by thyroid hormone is mediated by a DNA element in the first intron. J. Biol. Chem. 281:16870-16878.

Hanna SE, Wang HH. 2006. Environmental factors affect the expression of Listeria monocytogenes virulence factor inlA . J Food Protect. 69(11):2754-2757.

Blaschek PH, Wang HH, Agle M. (eds.) 2006. Biofilms in the Food Environment. Blackwell Publishing Co.

Hanna ES, Wang HH. 2006. Biofilm formation by Listeria monocytogenes . In Biofilms in the Food Environment. (Balschek HP, Wang HH, Agle M, eds.). Blackwell Publishing Co.

Manuzon MY, Wang HH. 2006. Mixed Culture Ecosystem. In Biofilms in the Food Environment (Balschek HP, Wang HH, Agle M, eds.). Blackwell Publishing Co.

Wang HH. 2006. Methodologies for genetic study and modification of bacteria: gram-positive bacteria. In Encyclopedia of Biotechnology in Agriculture and Food (Bridges A, Hoove DG, Wheeler MB, eds). Marcel Dekker. (Accepted).

Connor CJ, Luo H, McSpadden-Gardener BB, Wang HH. 2005. Development of a real-time PCR-based system targeting the 16S rRNA gene sequence for rapid detection of Alicyclobacillus spp. in juice products. Int. J. Food Microbiol. 99:229-235.

Luo H, Wan K, Wang HH. 2005. High-frequency conjugation system facilitates biofilm formation and pAMß1 transmission by Lactococcus lactis . Appl. Environ. Microbiol. 71:2970-2978.

Hanna SE, Connor CJ, Wang HH. 2005. Real-time polymerase chain reaction for the food microbiologist: technologies, applications, and limitations. J Food Sci. 70:49-53.

Pettipher GL, Jay JM, Wang H. 2005. Microbiological Techniques. In Encyclopedia of Analytical Science, Second Edition (Worsfold PJ, Alan Townshend A, Poole CF, eds.), Elsevier Science, Oxford , UK . Vol. 6, pp. 16-24.

Luo H, Yousef AE, Wang HH. 2004. A real-time polymerase chain reaction-based method for rapid and specific detection of spoilage Alicyclobacillus spp. in apple juice. Lett. Appl. Microbiol. 39:376-382 .

Marsh EJ, Luo L, Wang H. 2003. A three-tiered approach to differentiate Listeria monocytogenes biofilm-forming abilities. FEMS Microbiol Lett. 228:203-210.

 

 

Links

Department of Food Science and Technology

Department of Microbiology; The Ohio State University; 376 Bioscience Building; 484 West 12th Ave.; Columbus, Ohio USA; 43210-1292; Phone: 614-292-2301; Fax: 614-292-8120
Riffe Research Center

 

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Department of Microbiology
The Ohio State University
376 Biological Sciences Building
484 West 12th Ave.
Columbus, Ohio USA 43210-1292
Phone: 614-292-2301
Fax: 614-292-8120

The Ohio State University

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