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Stéphanie
Seveau, Ph.D.
Assistant
Professor
Departments
of Microbiology & Internal Medicine
Center
for Microbial Interface Biology
seveau.1@osu.edu
Room
541, Biological Sciences Building
Ohio
State University
484
West 12 th Avenue
Columbus
, OH 43210-1292
Tel:
(614) 247-7671
Fax:
(614) 292-8120
Education
1997
Ph.D., Pierre and Marie Curie University, Paris, France
1998-2001
Postdoctoral Training, Department of Biochemistry, Weill
Medical College of Cornell University, NewYork, USA
2001-2005
Research Investigator, Department of Cell Biology and
Infection, Pasteur Institute, Paris , France
2005-2006
Research Investigator, Department of Microbiology and
Immunology, University of Michigan Medical School, Ann
Arbor, Michigan, USA
RESEARCH
INTERESTS
Dr.
Seveau’s research is focused on understanding, at the
molecular level, host-pathogen interactions and the
innate immune responses to infection. The results of
her research provide a molecular basis for the logical
design of new anti-microbial therapeutics. Specific
projects currently are to:
1.
Establish how Listeria monocytogenes invades
and establishes infections of mammalian cells and tissues.
L. monocytogenes is a food-borne intracellular
bacterial pathogen that causes gastroenteritis, meningitis,
encephalitis and fetal infections of humans. L.
monocytogenes lives inside host cells, and its
ability to invade mammalian cells facilitates both its
initial transit through the epithelial barrier of the
digestive tract, and subsequent colonization of many
internal organs. State-of-the-art quantitative fluorescence
microscopy is used as the primary approach to investigate
and quantify L. monocytogenes infections of
live host cells. The complex dynamics of the signaling
events that lead to L. monocytogenes uptake
by epithelial cells and macrophages are being determined
and characterized.
2.
Determine the molecular basis, and outcome of host-cell
signaling induced by cholesterol-dependent cytolysin
(CDCs) toxins. CDCs are a family of closely-related
pore-forming bacterial toxins produced by many Gram-positive
Listeria, Streptococcus, Bacillus
and Clostridium species. At concentrations
below those that cause host-cell lysis, these virulence
factors function as signaling molecules but the underlying
molecular biology and the host cell responses to this
toxin-signaling remain unknown. To gain this information,
we are investigating the signaling
properties of listeriolysin O, a CDC and the major virulence
factor of L. monocytogenes. Structure-function
dissection of listeriolysin O is being combined with
live-cell imaging to establish how this toxin affects
host-cell signaling during the course of a bacterial
infection.
Recent
publications
Seveau,
S., Tham, T.N., Payrastre, B., Hoppe, A., Swanson, J.A.,
and Cossart, P. 2006.
Spatial
distribution of PI (3,4) P 2 and PI (3,4,5) P 3 phosphoinositides
is crucial for Rac1 activation in Met signaling pathway.
Cell. Microbiol. Manuscript In Press.
Martinez
, J.J., Seveau, S., Veiga-Chacon, E., Matsuyama , S.,
and Cossart, P. 2005. Ku70, a component
of DNA-dependent protein kinase, is a receptor involved
in Rickettsia conorii invasion of mammalian
cells. Cell 123 (6):1013-1023
Seveau,
S., Bierne, H., Giroux, S., Prevost, M.C., and Cossart,
P. 2004. Role of lipid rafts in E-cadherin-
and HGF-R/Met-mediated entry of Listeria monocytogenes
into host cells. J. Cell Biol. 166:743-753.
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