Circular Dichroism Spectropolarimeter

Using the Circular Dichroism Spectropolarimeter

CD Operating Instructions:

• Make sure liquid nitrogen level is above E-Mark.
  
• Turn "pressure build" valve on cylinder.
  
• Turn "gas use" valve on cylinder. The pressure should always be 30 psi.
  
• O₂ indicator (tube with silica and sand) should be brownish black.
  
• Open valves (blue) of snubber parallel to tube (these valves are below the comp.) first L.H.S. the R.H.S.
  
• Wait for 20 min.
  
• Turn lamp power on. See the N₂ indicators (blue mark should coincide with the N₂ indicator). When "Lamp ready" lights up, turn on the lamp.
  
• Record lamp hour. Allow lamp to warm up for 30 min (for better baseline)
  
• Turn CPU and Mac on. Type "yes" on PC to initiate the instrument.
  
• Start "Star Stationary" on Mac
  
• Use Quartz cuvettes and seal them with stoppers. Make sure there is no chemical residue in contact with the cuvette holder.
  

For Quitting:

• Transfer your raw data to your disc. It is now safe to turn off the power switch for the CPU
  
• Record the lamp hour. Turn off the power switch for the lamp
  
• Wait for 10 min to allow nitrogen gas to cool down the lamp
  
• Shut valves in reverse order
  
• Before you leave, make sure that the work area is neat and tidy.